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\nMethods for Trapping and Sampling Small Mammals for Virologic Testing [PDF \u2013 2 MB]
\nA Spanish version of the manual is available on the Pan American Health Organization\u2019s (PAHO) website.
\nThis manual is intended as a guide for those persons performing ecologic and epidemiologic studies involving populations of rodents which are potentially infected with hantavirus. However, the procedures outlined are appropriate for any study of small-mammal populations that may harbor an infectious zoonotic agent capable of causing severe disease or death.
\nThe manual covers the following major topics in detail: selection of appropriate collection sites; trapping methods that provide a representative sample of the rodent population; handling, operation, and placement of traps for small mammals; safe and humane techniques for trapping and handling rodents; selection of appropriate sample fluids and tissues and detailed methods for obtaining these samples; proper storage, packaging and shipment of specimens to the laboratory; effective decontamination and cleaning of traps and other materials; safe disposal of infectious wastes; and careful collection and recording of all pertinent data.
\nThe manual is illustrated with black and white photos to assist the trainee in understanding these techniques. Sample forms are provided in appendices and can be adapted to specific programs by users.
\nIt is our hope that the information contained in this manual will provide assistance to investigators involved in the collection and handling of small mammals and will facilitate surveillance efforts necessary to control and prevent the spread of infectious diseases.
\nWarning: The manual\u2019s text includes graphic photos of rodent necropsy procedures. These images may not be appropriate for viewing by children.
\nThe protocols described herein have been approved by the Animal Care and Use Committee at the Centers for Disease Control and Prevention. Investigators conducting research involving live animals should have their research protocols approved by their institutional animal care and use committee and should conduct all such research in accordance with federal guidelines (NIH 1985), the federal Animal Welfare Act (P.L. 89-544, as amended by P.L. 91-579 and P.L. 94-279) and Endangered Species Act (P.L. 93-205), and other applicable state and local laws, regulations, or policies.
\nThe manual contains information on packing and shipping specimens. However, updated information is available from two online sources:
\nHantavirus Pulmonary Syndrome (July 2011)
\nOf Mice and Man (January 2010)
\nCuando entran los ratones
\nPost it on your refrigerator! Nail it up in your shed or barn! Give a copy to your friends and neighbors!
\nThis handy card has quick tips and pictures so you\u2019ll always remember how to rodent-proof your home or clean up if you find rodents. It\u2019s available in both English and Spanish versions.
\nCommon household disinfectants can be used to substitute for bleach solution when spraying down areas infested by rodents. If you\u2019re worried that the surface to be sprayed will stain or discolor, detergent \u2014 not plain soap \u2014 can be used instead.
\nThe prevention card is provided here courtesy of the the New Mexico State Department of Health and New Mexico State University. Our thanks and appreciation to these institutions and to Mike Barnes, Robin Crabtree, and Leigh Ford.
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